Metanephrines reagents set
The Metanephrines reagents set from diagnotix • •• measures three components in plasma with the help of an LC-MS: metanephrine, normetanephrine en 3-methoxytyramine.
A deuterium-labeled derivative of all three metabolites is added to each sample during sample processing. After which the interfering substances are removed using SPE columns. A good chromatographic separation is obtained by using an amide column. The eluent of this can be immediately analyzed by the LC-MS.
The accuracy of the test is guaranteed by a 6-point calibration based on spiked 0 plasma. This is to simulate the samples’ matrix as much as possible. To continuously guarantee the quality of the test, it is possible to provide a 3-levels control with this same matrix.
Markers stated in the SKML surveys are recovered accurately.
- 6-point calibrator based on a 0-plasma matrix
- Internal standard with deuterium labeled metanephrine, normetanephrine en 3-methoxytyramine
- Good chromatographic separation
With a number of rare tumors too much adrenaline, noradrenaline and/or dopamine is released. In order to diagnose these tumors, these catecholamines need to be determined quantitatively. Because several factors in the body can influence this with both positive as negative outcomes as a result, the metabolite metanephrine, normetanephrine en 3-methoxytyramine are measured.