Metanephrines LC-MS/MS

Metanephrines LC-MS reagens kit

SKU: 1060 KIT M MET

The Diagnotix kit for the determination of Metanephrines in (EDTA) plasma/serum by LC-MS/MS.

Advantages of the Diagnotix Metanephrines LC-MS kit

Extreme robust semi-HILIC method
Excellent separation of all compounds including 3-MT
No hydrolysis (boiling) step necessary when measuring in blood

Intended Use

The Diagnotix Free Metanephrins LC-MS/MS is designed to determing the patient's metanephrine, nor metanephrine and 3-MT concentrations in blood as a basis for further diagnostics.

Technical Benefits

Based on a semi-HILIC method, thus easy baseline separation of all three compounds guaranteed.
Stable retention time, as this HILIC method is extremely robust.
Quantitative analyses of 3-MT is possible.
Due to the chromatographic stability you achieve high data quality and win time in data-analysis.
Check the technical details for the outstanding validation scores.

Why Choosing for blood sampling over urine sampling?

Urine samples require a hydrolysis step, which typically involves boiling. This process is time-consuming and often results in unpleasant odors. By transitioning to blood samples, the hydrolysis step is eliminated, streamlining sample preparation. This not only saves time but also creates a more efficient and user-friendly workflow for analysts.

Especially interesting for: Laboratories conducting frequent metanephrine testing and seeking to enhance efficiency while optimizing working conditions.

Contact us for More Information

Interested in learning more about our Metanephrines LC-MS analysis? Contact us via the contact form below. We looking forward to hear from you.

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The Diagnotix Metanephrines Reagent Set is able to accurately quantify the 3-MT group.

Components

Metanephrine
Normetanephrine
3-methoxytyramine (3-MT)

At a glance

Matrix: (EDTA) plasma
Technique: LC-MS/MS
Internal Standard: Metanephrine-D3, Normetanephrine-D3, 3-MT-D4

Included in kit (200 assays):

1061 CAL M MET, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 2 ml
1069 M MET, Internal Standard D3 D4, 4 x 6 ml
1070 M MET, Mobile Phase I, 1 x 250 ml
1071 M MET, Mobile Phase II, 2 x 500 ml
1072 M MET, Washing Solution 1, 2 x 220 ml
1073 M MET, Washing Solution 2, 2 x 220 ml
1074 M MET, Washing Solution 3, 2 x 100 ml
1078 M MET, Elution Solution, 2 x 30 ml
1079 M MET, Buffer, 4 x 30 ml
10601 M MET, Autosampler Washing Solution, 1 x 1000 ml

Clinical Relevance

The Free Metanephrines in (EDTA) plasma test measures three metabolites of the catecholamines epinephrine, norepinephrine and dopamine. These catecholamines are made in the adrenal gland to regulate the heartbeat, blood pressure and glucose concentrations. They can also be released in excess by rare tumors, such as pheochromocytomas and paragangliomas, on adrenal and extra-adrenal chromaffin tissue. The majority of these tumors release epinephrine and norepinephrine. Some rare tumors however excrete mostly, or sometimes solely, dopamine.

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Chromatograms

Baseline separation of all three compounds

Technical data

Applicable for at Least:	Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine
Limit of Quantification; Metanephrine / Normetanephrine / 3-MT (nmol/l)	0.032 / 0.041 / 0.093
Linearity; Metanephrine / Normetanephrine / 3-MT (µmol/l)	18 / 25 / 25
Accuracy	Passes (<15% CV)
Simple Precision; Metanephrine / Normetanephrine / 3-MT (CV %)	1.6 / 1.9 / 2.8
Complex Precision; Metanephrine / Normetanephrine / 3-MT (CV %)	6.1 / 5.3 / 5.6
Ionization	Electrospray
MS/MS mode	Positive
Sample Preparation	SPE
Preferred Column	Column Acquity UPLC BEH Amide 1.7µm 2.1 x 100mm
Analysis Time	4 minutes
Flow Rate	0.4 l/min
Injection Volume	5-20 µl
Validation Report Available	Yes

Please Note:

Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.

For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.