LCMS - Vitamin A & E

Vitamin A & E Reagens Set

SKU: 1080 KIT M VAE

Components

Vitamin A (Retinol)
Vitamin E (α-Tocopherol)

At a glance

Matrix: serum/plasma
Technique: LC-MS/MS
Internal Standard: Retinol-D6, α-Tocopherol-D3

Included in kit (300 assays):

2001 CAL HM VAE, Calibrator Set (Calibrator 1 – 6),
1090 M VAE, Deproteinization Solution with Internal Standard, 3 x 100 ml
1091 M VAE, Mobile Phase I, 1 x 250 ml
1092 M VAE, Mobile Phase II, 1 x 250 ml
1096 M VAE, Autosampler Washing Solution, 1 x 1000 ml

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Chromatograms

Technical data

Applicable for at Least:	Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine
Limit of Quantification; Retinol / α-Tocopherol (µmol/l)	0.052 / < 0.927
Linearity; Retinol / α-Tocopherol (µmol/l)	> 9 / 145
Accuracy	Passes (<15% CV)
Simple Precision; Retinol / α-Tocopherol (CV %)	1.6 / 1.6
Complex Precision; Retinol / α-Tocopherol (CV %)	2.7 / 3.4
Ionization	Electrospray
MS/MS mode	Positive
Sample Preparation	25 µl sample → 975 µl deprot + IS → centrifuge → inject
Preferred Column	Waters Xbridge BEH C18 1.7 µm 2.1 x 50mm
Analysis Time	4 minutes
Flow Rate	0.55 ml/min
Injection Volume	2 µl
Validation Report Available	Yes

Please Note:

Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.

For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.