Vitamin A&E LC-MS

Vitamin A & E LC-MS Reagens Kit

SKU: 1080 KIT M VAE

Advantages of the Diagnotix Vitamin A&E LC-MS kit:

40 times deproteinization solution instead of the usual 2-3 times, leading to increased column stability and lifespan.
Six Calibrator Levels in Human Serum
Easy to install onto your LC-MS system
No concern of coeluting other Tocopherols or Tocotrienols (See below the text 'Why transition from HPLC to LC-MS?' to understand why this is an important topic)

Components:

Vitamin A (Retinol)
Vitamin E (α-Tocopherol)

At a glance:

Matrix: serum/plasma
Technique: LC-MS/MS
Internal Standard: Retinol-D6, α-Tocopherol-D3

Intended use

The Diagnotix Vitamin A&E LC-MS kit is developed to determine the patient's Retinol and/or α-Tocopherol concentration in serum or plasma as a basis for further diagnostics.

Technical benefits

only 25 µl sample needed
No concern of coeluting any other vitamin E within our HPLC-assay. Commercial supplements, containing all vitamin E molecules, are widely available. This highlights the importance of using a robust HPLC-UV or fluorescence method for accurate analysis.
No need for additional HPLC systems in your lab. Easily integrate this method into your existing LC-MS platform.

Why moving over from HPLC to LC-MS?

Save time and reduce maintenance costs by eliminating your HPLC system.

While analyzing vitamins A and E by HPLC (UV or fluorescence) is relatively straightforward, it requires maintaining an HPLC system. If your LC-MS system is not fully utilized, transferring your vitamin A and E method to LC-MS could save you time and reduce maintenance costs.

No need to separate other tocopherols and tocotrienols, which could potentially lead to longer runtimes and reduced column lifespan.

Another compelling reason to switch to LC-MS is the growing availability of supplements containing a wide range of tocopherols and tocotrienols analogs in varying concentrations. These can elevate the levels of other tocopherols and tocotrienols in the patient's sample, all of which share similar hydrophobic properties. While HPLC can separate these compounds, it potentially extending sample run times and becomes increasingly challenging as the column ages. Thus your column life time might be drastically reduced.
In contrast, LC-MS eliminates the need for full chromatographic separation since the molecules differ in mass. This ensures no interference from other tocopherols or tocotrienols, while maintaining a fast and efficient sample-to-sample throughput.

An enhanced deproteinization step for greater stability and increased lifespan of your column.

As a final point, with the Diagnotix LC-MS solution, you perform a significant additional dilution (40 times) during the deproteinization step, removing even more protein compared to traditional HPLC methods (which typically use 2 to 3 times dilution). This further contributes to longer column stability and lifespan.

Especially interesting for laboratories that:

I. Runs large numbers of samples once or more times per month.
II. Or are looking to transition from HPLC to LC-MS to enhance operational efficiency and reduce overall maintenance costs.

Contact Us for More Information

Interested in learning more about our Vitamin A&E LC-MS solution? Contact us via the contact form below. We looking forward to hear from you!

Contact form

Webinar Modernizing LC-MS workflows: Case study overcoming Vitamin A&E challenges and embracing innovation

Included in kit (300 assays):

2001 CAL HM VAE, Calibrator Set (Calibrator 1 – 6),
1090 M VAE, Deproteinization Solution with Internal Standard, 3 x 100 ml
1091 M VAE, Mobile Phase I, 1 x 250 ml
1092 M VAE, Mobile Phase II, 1 x 500 ml
1096 M VAE, Autosampler Washing Solution, 1 x 1000 ml

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Chromatograms

Technical data

Applicable for at Least:	Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine
Limit of Quantification; Retinol / α-Tocopherol (µmol/l)	0.052 / < 0.927
Linearity; Retinol / α-Tocopherol (µmol/l)	> 9 / 145
Accuracy	Passes (<15% CV)
Simple Precision; Retinol / α-Tocopherol (CV %)	1.6 / 1.6
Complex Precision; Retinol / α-Tocopherol (CV %)	2.7 / 3.4
Ionization	Electrospray
MS/MS mode	Positive
Sample Preparation	25 µl sample → 975 µl deprot + IS → centrifuge → inject
Preferred Column	Waters Xbridge BEH C18 1.7 µm 2.1 x 50mm
Analysis Time	4 minutes
Flow Rate	0.55 ml/min
Injection Volume	2 µl
Validation Report Available	Yes

Please Note:

Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.

For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.