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LCMS - Nortriptyline

Nortriptyline Reagent Set

SKU: 4140 P NTP


  • Nortriptyline
  • Z-10-OH-nortryptiline
  • E-10-OH-nortyptiline

In het kort

  • Matrix: Serum
  • Technique: LC-MS/MS
  • Internal Standard: Clozapine-D4

Included in kit (300 assays)

  • 4141 CAL P NTP, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 500 µl
  • 4149 P NTP, Deproteinization Solution + Internal Standard, 3 x 100 ml
  • 4064 P PS1, Mobile Phase I, 1 x 500 ml
  • 4065 P PS1, Mobile Phase II, 1 x 500 ml
  • 4066 P PS1, Autosampler Washing Solution, 1 x 1000 ml

Tricyclic antidepressants (amitriptyline, nortriptyline, clomipramine, imipramine and desipramine) and antipsychotics (clozapine). Therapeutic Drug Monitoring (TDM) is based on the assumption that there is a relationship between the blood concentration and clinical effect (therapeutic improvement and adverse effects). It also assumes there is a concentration range of the drug which is characterized by maximal effectiveness and maximal safety, the “therapeutic window”. The Diagnotix kit for measuring tricyclic antidepressants (TCAs) and antipsychotics (PS1) includes the TCAs amitriptyline, nortriptyline, clomipramine, imipramine and desipramine (including the drugs’ metabolites) and the antipsychotic clozapine (including the metabolite norclozapine).

The TCAs amitriptyline, nortriptyline, clomipramine, imipramine and desipramine are used for the treatment of several types of depression, obsessive compulsive disorder, neuropathic pain, nocturnal enuresis, and for the prophylactic treatment of chronic tension type headache and migraine. TCAs have a pronounced inter-individual pharmacokinetic variability and a narrow therapeutic window. Studies on the relation between blood concentration and clinical improvement have supported the relation for the TCAs. Systematic reviews and meta-analyses led to convincing evidence of a significant relationship between clinical outcomes and plasma concentrations for the TCAs which are associated with a high probability of response.


Technische data

Applicable for at Least: Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine
Limit of Quantification; Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline (µg/l) 6.46 / 5.65 / 15.49
Linearity; Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline (µg/l) 1250 / 1050 / 288
Accuracy Passes (<15% CV)
Simple Precision; Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline (CV %) < 6.6 / < 4.0 / < 5.3
Complex Precision; Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline (CV %) < 10.6 / < 8.1 / < 8.1
Ionization Electrospray
MS/MS mode Positive
Sample Preparation 25 µl sample → 1 ml deprot + IS → centrifuge → inject
Preferred Column Acquity UPLC HSS T3 1,8 µm 2.1x100 mm
Analysis Time 7 min
Flow Rate 0.4 ml/min
Injection Volume 2-20 µl
Validation Report Available Yes

Please Note:

Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.

For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.

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