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LCMS - Nortriptyline
Nortriptyline Reagent Set
Components
- Nortriptyline
- Z-10-OH-nortryptiline
- E-10-OH-nortyptiline
At a glance
- Matrix: Serum
- Technique: LC-MS/MS
- Internal Standard: Clozapine-D4
Included in kit (300 assays)
- 4141 CAL P NTP, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 500 µl
- 4149 P NTP, Deproteinization Solution + Internal Standard, 3 x 100 ml
- 4064 P PS1, Mobile Phase I, 1 x 500 ml
- 4065 P PS1, Mobile Phase II, 1 x 500 ml
- 4066 P PS1, Autosampler Washing Solution, 1 x 1000 ml
Tricyclic antidepressants (amitriptyline, nortriptyline, clomipramine, imipramine and desipramine) and antipsychotics (clozapine). Therapeutic Drug Monitoring (TDM) is based on the assumption that there is a relationship between the blood concentration and clinical effect (therapeutic improvement and adverse effects). It also assumes there is a concentration range of the drug which is characterized by maximal effectiveness and maximal safety, the “therapeutic window”. The Diagnotix kit for measuring tricyclic antidepressants (TCAs) and antipsychotics (PS1) includes the TCAs amitriptyline, nortriptyline, clomipramine, imipramine and desipramine (including the drugs’ metabolites) and the antipsychotic clozapine (including the metabolite norclozapine).
The TCAs amitriptyline, nortriptyline, clomipramine, imipramine and desipramine are used for the treatment of several types of depression, obsessive compulsive disorder, neuropathic pain, nocturnal enuresis, and for the prophylactic treatment of chronic tension type headache and migraine. TCAs have a pronounced inter-individual pharmacokinetic variability and a narrow therapeutic window. Studies on the relation between blood concentration and clinical improvement have supported the relation for the TCAs. Systematic reviews and meta-analyses led to convincing evidence of a significant relationship between clinical outcomes and plasma concentrations for the TCAs which are associated with a high probability of response.
Chromatograms
Technical data
| Applicable for at Least: | Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine |
|---|---|
| Limit of Quantification; Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline (µg/l) | 6.46 / 5.65 / 15.49 |
| Linearity; Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline (µg/l) | 1250 / 1050 / 288 |
| Accuracy | Passes (<15% CV) |
| Simple Precision; Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline (CV %) | < 6.6 / < 4.0 / < 5.3 |
| Complex Precision; Nortriptyline / E-10-OH-Nortriptyline / Z-10-OH-Nortriptyline (CV %) | < 10.6 / < 8.1 / < 8.1 |
| Ionization | Electrospray |
| MS/MS mode | Positive |
| Sample Preparation | 25 µl sample → 1 ml deprot + IS → centrifuge → inject |
| Preferred Column | Acquity UPLC HSS T3 1,8 µm 2.1x100 mm |
| Analysis Time | 7 min |
| Flow Rate | 0.4 ml/min |
| Injection Volume | 2-20 µl |
| Validation Report Available | Yes |
Please Note:
Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.
For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.
More info?
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Contact details
De Plassen 4
9902 SE Appingedam
The Netherlands