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5-HIAA, VMA, HVA in Urine Reagent Kit (RUO)
5-HIAA, VMA, HVA in Urine Reagent Kit (RUO)
The Diagnotix Catecholamine Metabolites Reagent Kit is designed for quantitative LC-MS/MS analysis of the metabolites 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA) and vanillylmandelic acid (VMA) in human urine.
The kit provides a complete reagent set for laboratories conducting research on neurotransmitter and catecholamine metabolites using UHPLC-MS/MS systems. Matrix-matched calibrators and stable isotope-labelled internal standards support reliable quantification and help compensate for matrix effects during analysis.
The assay features straightforward sample preparation and a robust workflow, allowing the method to be easily implemented on existing LC-MS/MS platforms. This makes the kit particularly suitable for laboratories seeking a standardised approach without the need to develop a complete LC-MS/MS method in-house.
Calibrators are assigned using multiple independent SKML external quality assessment samples.
The reagent kit is supplied as a Research Use Only laboratory product.
Technical data
| Repeatability (Simple Precision) (CV%) | Control I (low) |
|---|---|
| 5-HIAA: 1.6 % | |
| VMA: 2.0 % | |
| HVA: 1.8 % | |
| Control III (high) | |
| 5-HIAA: 0.7 % | |
| VMA: 1.2 % | |
| HVA: 1.5% | |
| Pooled sample: | |
| 5-HIAA: 1.1 % | |
| VMA: 1.6 % | |
| HVA: 2.2 % | |
| Reproducibility (Complex Precision) (CV%) | Control I (low) |
| 5-HIAA: 2.5 % | |
| VMA: 4.2 % | |
| HVA: 3.2 % | |
| Control III (high) | |
| 5-HIAA: 4.0 % | |
| VMA: 4.3 % | |
| HVA: 3.9 % | |
| Pooled sample: | |
| 5-HIAA: 7.8 % | |
| VMA: 8.5 % | |
| HVA: 6.1 % | |
| Limit of Quantification | 5-HIAA: 0.7 µmol/l |
| VMA: <0.29 µmol/l | |
| HVA: <0.24 µmol/l | |
| Linearity | 5-HIAA: 300 µmol/l |
| VMA: 300 µmol/l | |
| HVA: 300 µmol/l |
More info?
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Contact details
De Plassen 4
9902 SE Appingedam
The Netherlands