Cortisol & Cortisone reagents set
The Cortisol & Cortisone reagents set from diagnotix • •• can determine the concentrations of both cortisol and cortisone in urine or saliva.
First an internal standard with D7 labeled cortisol and D8 labeled cortisone is added. All interfering substances are then removed using SPE columns, after which the eluent is evaporated to dryness. The residue is dissolved and can now be analyzed using the LC-MS.
The accuracy of the test is guaranteed with a 6-point calibration based on spiked humane 0 urine. This way the samples’ matrix can be simulated as much as possible. To continuously guarantee the quality of the test, it is possible to provide a 3-level control with this same matrix.
- 6-point calibrator based on humane spiked 0 urine
- Internal standard with deuterium labeled cortisol and cortisone
- Proper product clean-up using SPE-columns
- Extremely sensitive, making it possible even the very low concentrations in saliva can be measured
The ratio between cortisol and cortisone is of clinical importance with the diagnosis of Cushing syndrome. This even indicates apparent mineralocorticoid excess, a disease where the 11β-hydroxysteroid dehydrogenase-activity is weakened significantly or is even absent.