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LCMS - Immunosuppressants

Immunosuppressants Reagent Set

SKU: 4180 KIT P IMM

Components

  • Ciclosporine
  • Tacrolimus
  • Everolimus
  • Sirolimus

At a glance

  • Matrix: Whole blood
  • Technique: LC-MS/MS
  • Internal Standard: [2H12]-Cyclosporin A, [13C2,2H4]-Everolimus, [13C,2H3]-Sirolimus [13C,2H4]-Tacrolimus.

Included in kit (300 assays):

  • 4181 CAL P IMM, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 500 µl
  • 4189 P IMM, Deproteinization Solution + Internal Standard, 3 x 55 ml
  • 4190 P IMM, Zinc Sulphate Solution, 3 x 25 ml
  • 4191 P IMM, Mobile Phase I, 1 x 500 ml
  • 4192 P IMM, Mobile Phase II, 1 x 500 ml
  • 4196 P IMM, Autosampler Washing Solution,1 x 1000 ml

Patients who receive a solid organ transplantation (SOT) or a stem cell transplantation (SCT) require immunosuppressant drugs to suppress the patients’ immune response to the transplant and prevent rejection of the transplant. The goal with immunosuppression is to induce donor specific tolerance for the transplant with minimal impairing of the patients’ defenses or increasing the susceptibility to infections. The most common immunosuppressants prescribed are the calcineurine inhibitors cyclosporine and tacrolimus, and the mTOR inhibitors sirolimus and everolimus. These immunosuppressants are used for the prophylaxis and treatment of graft rejection following SOT (kidney, lung, hart, and liver) and SCT. With cyclosporine, tacrolimus, sirolimus, and everolimus, clinical variability due to inter- and intra- patient pharmacokinetic variability is seen. This pharmacokinetic variability, in combination with an excellent correlation between blood concentrations and efficacy/toxicity of the treatment and the narrow therapeutic range of the immunosuppressive drugs, makes monitoring of blood concentrations a crucial part of the treatment. The therapeutic range for each immunosuppressant drug depends on post-transplant time, concomitant immunosuppressive medication, and immunological risk.

Chromatograms

Technical data

Applicable for at Least: Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine
Limit of Quantification; Cyclosporin A / Everolimus / Sirolimus / Tacrolimus TBD
Linearity; Cyclosporin A / Everolimus / Sirolimus / Tacrolimus (µmol/l) 5000 / 180 / 200 / 190
Accuracy Passes (<15% CV)
Simple Precision; Cyclosporin A / Everolimus / Sirolimus / Tacrolimus (CV %) 2.1 / 5.1 / 5.5 / 3.8
Complex Precision; Cyclosporin A / Everolimus / Sirolimus / Tacrolimus (CV %) 5.0 / 7.9 / 5.5 / 5.9
Ionization Electrospray
MS/MS mode Positive
Sample Preparation 50 µl sample → 200 µl Zinc Sulphate → 500 µl deprot + IS → centrifuge → inject
Preferred Column Waters XBridge BEH C18 2,5µm 2,1x75mm XP
Analysis Time 4 minutes
Flow Rate 0.5 ml/min
Injection Volume 25 µl
Validation Report Available Yes

Please Note:

Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.

For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.

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Contact details

De Plassen 4
9902 SE Appingedam
The Netherlands

info@diagnotix.com +31 (0)596 20 10 62