Vitamin B1 & B6 reagents set
The Vitamin B1 & B6 reagents set from diagnotix • •• is based on Waters’ published TCA method – market leader in mass spectrometry. The method contains a lower TCA concentration which benefits your system.
- Vitamin B1 (Thiamine diphosphate)
- Vitamin B6 (Pyridoxal-5’-phosphate)
At a glance
- Matrix: Whole blood
- Technique: LC-MS/MS
- Internal Standard: Thiamine diphosphate-D3, Pyridoxal-5’-phosphate-D3
Included in kit (300 assays):
- 1022a CAL M VB1B6, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 250 µl
- 1029 M VB1B6, Internal Standard D3, 3 x 6 ml
- 1030 M VB1B6, Deproteinization Solution, 3 x 46 ml
- 1031 M VB1B6, Mobile Phase I, 1 x 500 ml
- 1032 M VB1B6, Mobile Phase II, 1 x 250 ml
- 10201 M VB1B6, Autosampler Washing Solution, 1 x 1000 ml
Water-soluble B Vitamins are important cofactors in cell metabolism. Two water-soluble vitamins with clinical relevance are Vitamins B1 and B6. Thiamine Diphosphate (TDP) is the biologically active form of Vitamin B1 and is required for various metabolic functions. Prolonged deficiency can cause Beriberi, a debilitating neurological disease. Pyridoxal 5- phosphate (PLP) is the biologically active form of Vitamin B6 and is a coenzyme for a number of transamination reactions. It plays critical roles in chronic disease and pro-inflammatory response. Additionally, both Vitamin B6 and B1 have also been linked to increased survival rates in the elderly.
|Applicable for at Least:||Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine|
|Limit of Quantification; Vitamin B1 / Vitamin B6 (µmol/l)||6.43 / 8.00|
|Linearity; Vitamin B1 / Vitamin B6 (µmol/l)||8000 / 3000|
|Accuracy||Passes (<15% CV)|
|Simple Precision; Vitamin B1 / Vitamin B6 (CV %)||2.4 / 3.4|
|Complex Precision; Vitamin B1 / Vitamin B6 (CV %)||4.7 / 4.9|
|Sample Preparation||50 µl Whole blood (EDTA and Heparin) → 50 µl IS → 400 µl deprot → shaker → centrifuge → inject|
|Preferred Column||Xbridge BEH C18 XP Column 2.5 µm, 3mm x 75 mm|
|Analysis Time||2.5 minutes|
|Flow Rate||0.6 ml/min|
|Injection Volume||10-20 µl|
|Validation Report Available||Yes|
Information as presented freely on this website is not sufficient for proper operation of these medical devices. Please refer to the applicable instructions for use and all accompanying information such as warning notices. All necessary documentation can be found in the download centre section of this website.
For other LC-MS/MS systems than listed above, please contact us to discuss the possibilities.