LCMS - Methylmalonic acid
Methylmalonic Acid Reagents Set
- Methylmalonic Acid
At a glance
- Matrix: serum/plasma
- Technique: LC-MS/MS
- Internal Standard: Methylmalonic Acid-D3
Included in kit (300 assays):
- 1001 CAL M MMA, Calibrator Set (Calibrator 1 – 6), 6 x 2 x 500 µl
- 1008 M MMA, Internal Standard, 3 x 6 ml
- 1009 M MMA, Deproteinization Solution, 3 x 55 ml
- 1010a M MMA, Mobile Phase I, 1 x 500 ml
- 1011 M MMA, Mobile Phase II, 1 x 300 ml
- 1012 M MMA, Solvent, 3 x 30 ml
- 10001 M MMA, Autosampler Washing Solution, 1 x 1000 ml
Methylmalonic acid is an organic acid of which the blood levels are usually raised in case of a B12 deficiency. Adenosylcobalamin – one of the two active forms of B12 – is a cofactor of the enzyme L-methylmalonyl-CoA- mutase, which converts L-methylmalonyl-Coa into succinyl-CoA. If adenosylcobalamin is lacking, excess D-methylmalonyl-CoA (precursor of Lmethylmalonyl-CoA) is converted into methylmalonic acid(MMA) which causes raised blood levels of MMA. In short: a B12 deficiency (usually) causes high MMA. High MMA serum values are also found in people with renal insufficiency, hypovolemia (decreased volume of circulating blood) and intestinal bacterial overgrowth. In these cases MMA levels cannot be used to diagnose B12 deficiency though a B12 deficiency might simultaneously exist. In the case of kidney disease (or hypovolemia) MMA levels in urine can be tested.
|Applicable for at Least:||Waters TQ-Sµ, Shimadzu LC-8050, Sciex Citrine|
|Limit of Quantification (nmol/l)||33.4|
|Accuracy||Passes (<15% CV)|
|Simple Precision (CV %)||2.0|
|Complex Precision (CV %)||4.9|
|Sample Preparation||100 µl sample → 50 µl IS → 400 µl deprot → centrifuge → evaporate/reconstitute → inject|
|Preferred Column||Acquity UPLC HSS T3 100 A 1,8 µm 2,1 mm x 100 mm|
|Analysis Time||6 minutes|
|Flow Rate||0.2 ml/min|
|Injection Volume||10-20 µl|
|Validation Report Available||Yes|
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Included in this set
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